Runs uploaded in manual mode have their info straight passed to bcl2fastq without having even more processing.
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Set A Index Adapters Check with Index 2 (i5) Orientation For more info on how to enter i5 bases over the sample sheet in forward or reverse complement orientation.
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Established B Index Adapters Consult with Index two (i5) Orientation for more information regarding how to enter i5 bases on the sample sheet in ahead or reverse enhance orientation.
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For Community Operate Supervisor (LRM), input the sequences during the reverse enhance when starting the operate And so the software immediately makes the ahead orientation as needed for Investigation. This can be the alternative of almost all of our guidance and is also just for the MiniSeq Fast kits.
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For operates uploaded manually (i.e., an Illumina Software isn't being used to produce the sample sheet), the index orientation is dependent upon which instrument that you are using. Please begin to see the Index Orientation Desk for info on ways to enter the i5 sequence based on the instrument and software package applied.